Scientist I - CRISPR, mammalian cells in Cambridge, MA at Apex Life Sciences

Date Posted: 7/20/2018

Job Snapshot

  • Employee Type:
  • Location:
    Cambridge, MA
  • Job Type:
  • Experience:
    Not Specified
  • Date Posted:
  • Job ID:

Job Description

Apex Life Sciences is seeking a highly motivated and skilled Scientist I to join a top Molecular Oncology team based in Cambridge.


  • Responsible for developing and executing molecular and cellular assays and methodologies to support target validation projects as well as drug discovery programs.
  • Also responsible for developing, optimizing and implementing functional genomic assays and potentially screen assays for novel target discovery.


  • BS degree in biological science or related field required; Master's degree preferred
  • 5+ years of experience in the area of molecular and cellular biology
  • Cell culture of adhesion and suspension cells( including primary cell culture).
  • Cell based assays (proliferation, apoptosis, cytotoxicity; luciferase reporter assays, Elisa western blot assay).
  • DNA and RNA isolation, PCR, RT-PCR and qPCR.
  • Molecular cloning (recombinant DNA cloning, PCR, plasmid preparation, DNA sequencing analysis, site directed mutagenesis).
  • Genome editing, Using CRISPR/Cas9 system to KO or KI gene in mammalian cells.
  • Design and perform siRNA transfection; inducible shRNA lentivirus infection and rescue with shRNA resistant cDNA.
  • Generation Lentivirus vector, packaging virus, virus titer, transduction mammalian cells and establish stable transgenic line.
  • Experience in cell imaging
  • Must be a strong team player with excellent oral and written communication skills and be able to work independently to design experiments, interpret data, and troubleshoot.

***For immediate consideration, please email your updated resume to with 'Scientist 869032' in the the subject line.

EEO Employer

Apex is an Equal Employment Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law. Apex will consider qualified applicants with criminal histories in a manner consistent with the requirements of applicable law. If you have visited our website in search of information on employment opportunities or to apply for a position, and you require an accommodation in using our website for a search or application, please contact our Employee Services Department at or 844-463-6178.