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Cell Line and Cell Culture Technical Specialist in Andover, MA at Apex Life Sciences

Date Posted: 11/28/2018

Job Snapshot

Job Description


DUTIES

  • Utilize molecular biology techniques to genetically engineer mammalian cell lines by overexpressing, knocking down or knocking out various gene targets.
  • Evaluate the engineered cells for the desired genotype and phenotype using shake flask and small scale bioreactor experiments

Must have thorough knowledge of molecular and mammalian cell biology including: 

  • Construction and verification of complex DNA vectors
  • Generation and characterization of genetically engineered cell lines
  • Knock-down or knock-out of gene targets using miRNA, siRNA, and CRISPR / Cas9 technology
  • Assess gene expression using RT qPCR
  • Perform western blot analysis to query the protein expression of various target genes.
  • When required, the candidate will also be working closely with the scientists to perform cell maintenance and conduct cell culture experiments in shakeflasks, bioreactors, miniature bioreactors (ambr15) etc.

SKILLS

  • BS/BA, MS/MA or PhD/DSc in Chemical Engineering, Biotechnology, Biochemical Engineering, Microbiology, Cell Biology, Biochemistry, Biology or equivalent fields, 0-2 years industrial experience preferred.
  • DNA ligation, Gibson assembly and Gateway assembly
  • In silico analysis of DNA (ex. vector NTI software)
  • Perform aseptic mammalian cell culture, 
  • Experience in performing molecular biology assays including but not limited western blots, qRT-PCR, etc
  • Experience with molecular cloning techniques such as plasmid construction, preparation and analysis, RNA, cDNA and genomic DNA preparation, sequencing, CRISPR/CAS9 technology 
  • Experience performing transfections to overexpress, knockout or knockdown genetic engineering targets. 
  • Ability to communicate effectively with scientists, compile results in electronic note books and prepare slides/reports on the experimental work performed as and when required. 
  • Course work and/or past experience in the cellular metabolism is preferred.

***For immediate consideration, please email your updated resume to Ljohnson@apexlifesciences.com with 'Cell Culture Technical Specialist' in the subject line.

EEO Employer

Apex is an Equal Employment Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, age, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law. Apex will consider qualified applicants with criminal histories in a manner consistent with the requirements of applicable law. If you have visited our website in search of information on employment opportunities or to apply for a position, and you require an accommodation in using our website for a search or application, please contact our Employee Services Department at 844-463-6178.




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